The Mitochondriotropic DecylTPP Group Lowers Mitochondrial OXPHOS Supercomplex Levels and Induces a Glycolytic Switch

Prof. Werner Koopman, from the Department of Biochemistry, Radboud Institute for Molecular Life Sciences, will join the Targeting Mitochondria 2021 Congress and will present a talk entitled "The Mitochondriotropic DecylTPP Group Lowers Mitochondrial OXPHOS Supercomplex Levels and Induces a Glycolytic Switch".

Prof. Koopman will discuss the bioenergetic effects of mitochondria-targeted Trolox(MitoE10), in comparison with mitochondria-targeted ubiquinone (MitoQ10) and two mitochondria-targeting moieties (decylTPP, TPMP). Incubation with these molecules did not greatly affect cell viability of primary human skin fibroblasts (PHSFs) from healthy individuals and a patient with Leigh Syndrome (LS). Unexpectedly, MitoE10, MitoQ10 and decylTPP reduced the levels and activity of the first oxidative phosphorylation complex (complex I or CI) and of the amount of  CI+CIII-containing OXPHOS super complexes. Functionally, these effects were associated with reduced cellular oxygen consumption rates, increased extracellular acidification rates , a less negative mitochondrial membrane potential (deltaPSI), a decreased mitochondrial size and elevated levels of hydroethidine-oxidizing ROS. Collectively, these results demonstrate that MitoE10, MitoQ10 and decylTPP lower the levels of CI and CI-containing super complexes in PHSFs, leading to mitochondrial dysfunction and glycolytic switch induction.

Targeting Microbiota 2021 Congress
October 20-22, 2021 - Paris, France & Online
www.targeting-mitochondria.com